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anti-cd21 (clone: 4e3)  (Thermo Fisher)


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    Thermo Fisher anti-cd21 (clone: 4e3)
    Anti Cd21 (Clone: 4e3), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti-cd21 (clone: 4e3)/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    anti-cd21 (clone: 4e3) - by Bioz Stars, 2026-02
    90/100 stars

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    GL7− Breg cells derive from CD21hi MZP B cells. (A, B) Splenic <t>CD21−/lo,</t> CD21hiCD23+ (marginal zone precursor, MZP B cells) and CD21hiCD23− (marginal zone, MZ B cells) B cells from .from wild-type (A–C) or CD19-deficient mice (C) were stimulated for 3 days with LPS (1 μg/ml) and analyzed by the intracellular cytokine-staining assay. Quadrants indicate percentage of IL-10-expressing GL7− and GL7+ B cells (A, C). Statistical analysis of the percentages of GL7−IL-10+B and GL7+IL-10+B cells in CD21−/lo, CD21hiCD23+ and CD21hiCD23− B cells (B). Data represent 3 independent experiments. (B) Data were analyzed by Student’s t-test (two tailed). Error bars, s.e.m. *P < 0.05, ****P < 0.0001.
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    GL7− Breg cells derive from CD21hi MZP B cells. (A, B) Splenic CD21−/lo, CD21hiCD23+ (marginal zone precursor, MZP B cells) and CD21hiCD23− (marginal zone, MZ B cells) B cells from .from wild-type (A–C) or CD19-deficient mice (C) were stimulated for 3 days with LPS (1 μg/ml) and analyzed by the intracellular cytokine-staining assay. Quadrants indicate percentage of IL-10-expressing GL7− and GL7+ B cells (A, C). Statistical analysis of the percentages of GL7−IL-10+B and GL7+IL-10+B cells in CD21−/lo, CD21hiCD23+ and CD21hiCD23− B cells (B). Data represent 3 independent experiments. (B) Data were analyzed by Student’s t-test (two tailed). Error bars, s.e.m. *P < 0.05, ****P < 0.0001.

    Journal: Molecular immunology

    Article Title: Pre-existing CD19-independent GL7 − Breg cells are expanded during inflammation and in mice with lupus-like disease

    doi: 10.1016/j.molimm.2016.01.011

    Figure Lengend Snippet: GL7− Breg cells derive from CD21hi MZP B cells. (A, B) Splenic CD21−/lo, CD21hiCD23+ (marginal zone precursor, MZP B cells) and CD21hiCD23− (marginal zone, MZ B cells) B cells from .from wild-type (A–C) or CD19-deficient mice (C) were stimulated for 3 days with LPS (1 μg/ml) and analyzed by the intracellular cytokine-staining assay. Quadrants indicate percentage of IL-10-expressing GL7− and GL7+ B cells (A, C). Statistical analysis of the percentages of GL7−IL-10+B and GL7+IL-10+B cells in CD21−/lo, CD21hiCD23+ and CD21hiCD23− B cells (B). Data represent 3 independent experiments. (B) Data were analyzed by Student’s t-test (two tailed). Error bars, s.e.m. *P < 0.05, ****P < 0.0001.

    Article Snippet: The following antibodies were purchased from eBioscience: fluorescence-conjugated anti-mouse CD3 (clone no. 145-2C11), CD4 (clone no. GK1.5), B220 (clone no. RA3-6B2), CD19 (clone no. MB19-1), CD5 (clone no. 53-7.3), CD1d (clone no. 1B1), IL-10 (clone no.JES5-16E3), Foxp3 (clone no.NRRF-30), IFNγ (clone no.XMG1.2), IL-17 (clone no. 17F3), CD21 (clone no.4E3) and CD23 (clone no. B3B4), GL7 (clone no. GL-7), IgG (Cat no. 11-4011-85) and IgM (clone no. RMM-1) antibodies.

    Techniques: Staining, Expressing, Two Tailed Test

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells

    doi: 10.1016/j.celrep.2020.108403

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Rat anti-CD21/CD35 (clone: 4E3) FITC , Thermo Fisher Scientific , Cat# 11-0212-82; RRID:AB_464976.

    Techniques: Activation Assay, Recombinant, Adjuvant, Plasmid Preparation, PCR Cloning, cDNA Synthesis, SYBR Green Assay, Sequencing, Software